Fluorescence lifetime imaging microscopy (FLIM): a non-traditional approach to study host-microbial symbioses

P Deore; I Wanigasuriya; SJ Tsang Min Ching; DR Brumley; MJH Van Oppen; LL Blackall; E Hinde

Journal article

Fluorescence lifetime imaging microscopy (FLIM): a non-traditional approach to study host-microbial symbioses

P Deore, I Wanigasuriya, SJ Tsang Min Ching, DR Brumley, MJH Van Oppen, LL Blackall, E Hinde

Microbiology Australia | Published : 2022

DOI: 10.1071/MA22008

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Abstract

Corals and their photosynthetic endosymbiotic algae (Symbiodiniaceae) produce a strong autofluorescent signal that spans the visible to near-infrared (NIR) spectrum. However, this broad-spectrum emission hinders the use of fluorescence in situ hybridisation (FISH) for the study of bacterial heterogeneity within the different niches of corals and Symbiodiniaceae, because FISH fluorophores also fluoresce within the visible to NIR spectrum. A solution to this impediment is to use fluorescence lifetime imaging microscopy (FLIM). The 'lifetime' property of fluorophores is a feature that enables sample (e.g. coral/Symbiodiniaceae) autofluorescence to be distinguished from FISH-labelled bacteria. I..

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University of Melbourne Researchers

Douglas Brumley Author

Madeleine van Oppen Author

Linda Blackall Author

Elizabeth Hinde Author

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Grants

Awarded by Gordon and Betty Moore Foundation

Funding Acknowledgements

PD, STMJ; DRB, MvO, LLB and EH are supported by the Gordon Betty Moore foundation (project number -9351); IW was supported by an Australian Research Council Discovery Project (DP180101387); DRB was supported by an Australian Research Council Discovery Early Career Researcher Award (DE180100911) and a Gordon & Betty Moore Foundation Symbiosis in Aquatic Systems grant; IW was supported by an Australian Research Council Discovery Project (DP180101387); EH is supported is supported by an Australian Research Council Future Fellowship (FT200100401) and MJHvO by an Australian Research Council Laureate Fellowship (FL180100036).